I j pharmacy life sciences identification and estimation. With the help of camag video store system camag switzerland. All methods and hptlc data fingerprints can be viewed on the screen or downloaded in pdf format. Merck, camag linomat v automatic sample applicator camag, camag 100. Camags modern software concept ensures the reliable operation of our hptlc systems and the documentationstorage of operating parameters and results as required by the customer. Hptlc hptlc is the improved method of tlc which utilizes the conventional technique of tlc in more optimized way it is also known as planar chromatography or flat bed. Suited for object formats up to about 16 x 21 cm 20 x 10 cm and 10 x 10 cm tlc hptlc plates. The objective of the present study is to evaluate the phytochemical composition and hptlc finger print profile of medicinally useful plant r. High performance thin layer chromatography hptlc presented by mr. The product, widely used in the pharmaceutical and chemical industries in purification processes, is of excellent quality. Hptlc is accurate, precise and reproducible, as proven by the method validation.
Analysis was performed on a camag hptlc system containing camag linomat 5, camag tlc scanner 3 with cats softwarewin. High performance thin layer chromatography hptlc and high. Camag hptlc system, consisting of linomat v spotting device and scanner iii with win cats 4 software stationary phase. Camag is in the final stage of developing the new generation software. The software option 21 cfr part 11 en ables customers to work in a controlled environment and to establish regula torycompliant processes. The peak purity of quercetin was assessed by comparing the. High performance thin layer chromatography hptlc nc silica f254 10. Densitometry was carried out with a camag tlc scanner3, fitted with win cats 1. Rapid hptlc ms identification of synthesis products. Merck using a camag linomat 5 sample applicator switzerland.
Comparison between hplc and hptlc densitometry for the. Fingerprint profile of an important therapeutic plant. Electronic signature processes and a set of log files. The coordinates are displayed during manual positioning and can be transferred into the. Introduction to modern thinlayer chromatography method development hptlc for the analysis of phytopharmaceuticals hptlc for the pharmaceutical. Hptlc high performance thin layer chromatography is a sophisticated and automated form of tlc.
The samples after extraction and drying redissolved with 1 ml of chloroform. Hptlc chromatograms are captured images of the plates under white or ultraviolet light. Scanning before derivatization, the plate was fixed in scanner stage camag tlc scanner 3 and scanning was done at uv 254nm. A number of enhancements can be made to the basic method of thinlayer chromatography to automate the different steps, to increase the resolution achieved and to allow more accurate quantitative measurements. Highperformance thinlayer chromatography wikipedia. Hptlc plates precoated with silica gel gf on aluminum tlc plate, 10cm x 10cm were from merck.
Hptlc study hptlc precoated, silica gel g 60 f25 merck. A specific chromogenic reagent for the detection of. Once you merge pdfs, you can send them directly to your email or download the file to our computer and view. It is also widely available in tribal belt of chhattisgarh. Issn tlc and hptlc fingerprint profiles of different. An external file that holds a picture, illustration, etc. Pdf a validated highperformance thinlayer chromatography. Hptlc format and layer 20 x 10 cm, 200 m layer thickness.
Densitometric detection was performed with a camag tlc scanner 3 camag, muttenz, switzerland installed with wincats. Hptlc principle, instrumentation and software abhishek. The sample were spotted in the form of bands with a camag 100microlite sample syringe hamilton, bondouz, switzerland syringe on. The coordinates are displayed during manual positioning of the stage and can be transferred into the program by. It is a major advancement of tlc principle with short time duration and better resolution. Hptlc method was conducted using an hptlc system with a developed mobile phase. Hptlc is a planar chromatography were the solvent moves through a plate fixed stationary phase, due to capillarity. It is a comprehensive collection of 100 methods for the identification of botanicals. The hptlc analysis was performed on aluminium plates precoated with silica gel 60 f 254, purchased from emerck, germany. Nov 29, 2017 hptlc hptlc is the improved method of tlc which utilizes the conventional technique of tlc in more optimized way it is also known as planar chromatography or flat bed chromatography principle.
Camag has a comprehensive quality system according to iso 9001. Camag has manufactured aluminium oxide since 1959 in switzerland. A number of enhancements can be made to the basic method of thinlayer chromatography to. The sample were spotted in the form of bands with a camag 100microlite sample syringe hamilton, bondouz, switzerland syringe on precoated silica gel aluminium plate 60f 254 20x10 with 250m thickness. The obtained data can be edited, archived and evaluated. Each method has a corresponding reference image that can be used to determine the identity of your botanical sample. The peak purity of quercetin was assessed by comparing the spectra at peak start, peak apex and peak end positions of the spot. Camag hptlc system equipped with automatic tlc sampler iv, tlc scanner 3, reprostar 3 with 12 bit ccd camera for photo documentation, wincats.
Phytochemical screening and hptlc fingerprint analysis of. Assessment of aflatoxin b1 in livestock feed and feed. The easy to navigate user interface effectively guides the user through the chromatographic process from definition of samples and substances to reporting of results. These format definitions are used in this catalog as well as in all camag literature. Camag hptlc system equipped with automatic tlc sampler iv, tlc scanner 3, reprostar 3 with 12 bit ccd camera for photo documentation, wincats planer chromatography software. The report will produce printable documents of your analysis as well as the possibility to move and save the data of the analysis to different file types like pdf, excel, word report settings. It can be applied to samples where analyte lack a convenient chromophore. Camag hptlc system equipped with a sample applicator linomat iv, twin trough. Spectrodensitometric scanning was performed by tlc scanner iii camag in. Hptlc is an improved version of tlc, while hplc is a pump driven flow system. Once files have been uploaded to our system, change the order of your pdf documents. Hptlc finger print profile of nhexane extract of rauwolfia.
Merck, darmstadt, germany using a camag linomat v sample applicator. The coordinates are displayed during manual positioning of the stage and can be transferred into the program by mouseclick. An introduction to hptlc and hptlcms waters corporation. The camag hptlc system was used for the finger print profiling using the mobile phase toluene. Phytochemical and hptlc studies of methanolic extract of. Pdf a simple, sensitive, precise and robust highperformance thinlayer.
L sample syringe hamilton, switzerland with a linomat 5 applicator camag, switzerland. Hptlc was performed with a camag muttenz, switzerland linomat v sample applicator, a camag twin trough tlc chamber, a camag tlc scanner 3, camag wincats software v 4. High performance thin layer chromatography hptlc and. Camag laboratory services is also your partner when it comes to education and training in the field of thinlayer chromatography. Hptlc fingerprint enables a particular plant to be identified and distinguished from closely related species. Drug standards and samples were applied on the hptlc plates using linomat 5 camag applicator under nitrogen gas flow. Calibration in tlchptlc using the michaelismenten function. Development of hptlc method and its validation for the. In muttenz we offer courses on the following subjects. The plate was removed from the chamber, dried in air and sprayed with 5% sodium hydroxide solution followed by 0. Hptlc analysis was performed on a camag hptlc system. Highperformance thinlayer chromatography hptlc is an enhanced form of thinlayer chromatography tlc. Various parts of the plant or the plant as a whole is used as treatment of so.
This solution was used as a test solution for hptlc analysis. Alternatively, methods and data compatible with visioncats camag software are also available. Free choice of application patterns on planar media of any format. All steps in the in the procedure are managed by the camag tlc software. The software organizes the workflow of hptlc, controls the involved camag hptlc pro modules and camag instruments, and manages data. High performance thin layer chromatography hptlccamag 14. Hptlc camag linomat v applicator, camag twin trough tlc chamber, camag tlc visualiser, camag wincats software and hamilton syringe 100 l standards and chemicalreagents all chemicals and reagents including methanol, isopropyl alcohol, ammonia, acetone, were of emerck and of analytical grade. Argemone mexicana mexican poppy is a species of poppy found in mexico and now widely naturalized in many parts of the world. Standard solution of quercetin and sample solution were applied to the plates as.
Hptlc analysis the samples were deposited in the form of bands of width 6 mm with a camag 100 microlitre sample syringe hamilton bonaduz, switzerland on precoated hptlc plate silica gel 60 f. Hptlc method development and validation of cefpodoxime. Fourier transformed infrared spectrophotometer ftir shimadzu 8400s 8. For electronic image acquisition a digital camera captures visible polychromatic light. Hence, hptlc had been considered due to its decisive advantages. Suited for object formats up to about 16 x 21 cm 20 x 10 cm and 10 x 10 cm tlchptlc plates. Available downloadable files always represent the most current. All steps in the in the procedure are managed by camag hptlc software. Hptlc is a valuable analytical tool for the investigation of herbal products and drugs. It is possible to get useful qualitative and quantitative information from the developed tlc plate14. Chromatographic conditions mixture of glacial acetic acid, nhexane, ethyl acetate and toluene 0. Pdf fundamentals and theory of hptlcbased separation. Microsyringe linomat syringe, hamiltonbonaduz schweiz, precoated silica gel 60 f 254 glass plates 10.
Hptlc method development and validation journal of. New information is continuously added to the atlas and existing information updated. Camag, switzerland saturated with the mobile phase. Tlc aluminum sheets silica gel 60 f 254 pre coated layer 20 cm x 10. Currently, camag is designing the next generation of analytical instruments for the highperformance thinlayer chromatography hptlc in addition, camag provides an automated highthroughput elution instrument dbsms 500 to the clinical diagnostics industry make new connections. Subsequent to the development, hptlc plates were dried in current of air with the help of air dryer in a wooden chamber. Hptlc study4,5 of methanolic extracts of the individual ingredients, inhouse formulation and marketed formulation were carried out along with the different marker. Standard solution of quercetin and sample solution were applied to the plates as bands 8. Camag tlc scanner 4 for the densitometric evaluation of tlc hptlc plates, spectral range 190 to 900 nm, plate sizes up to 20 x 20 cm, absorbance and fluorescence mode, camag visioncats controlled 028. The r f values and colour of the resolved bands were noted. The optimized chamber saturation time for mobile phase was 30 min at room temperature 24c2 at relative humidity of 605%. Camag has everything your lab may need for thinlayer chromatography. Pdf quantitative analysis and validation of method using hptlc.
L syringe hamilton, bonaduz, switzerland which was programmed using. We provide competent customer support and technical service for our products as well as education, training, application support and a range of contract laboratory services. Development and validation of hptlc stability indicating. Click here to see the list of methods included in the collection. High performance thin layer chromatography hptlc is the most powerful.
Hptlc plates provide improved resolution, higher detection sensitivity, and improved in situ quantification and are used for industrial pharmaceutical densitometric quantitative analysis. The method may be applied to identify the plant of ficus nervosa from other species. L sample syringe hamilton, camag twin trough chamber 10. In addition, hptlc analysis is rather simple and can be employed for multiple sample analysis. Tlc aluminum sheets silica gel 60 f 254 pre coated layer 20 cm x 10 cm, thickness 0. Highperformance thinlayer chromatography hptlc dayone.
Rearrange individual pages or entire files in the desired order. Camag tlc scanner 4 camag camag tlc hptlc, dbs, alox. A highperformance thin layer chromatography hptlc method. Hptlc system used for analysis was of camag hptlc system. The sample preparation is simple and the development time is short 4 min. Samples were applied using a linomat 5 camag, muttenz, switzerland semiautomated applicator with a 100. The peak table, peak display and peak densitogram were noted. Hptlc plate after elution of zones with the camag tlcms interface 2 c. Camag tlc visualizer 2 documentation system with 16 mm lens. Camags modern software concept ensures the reliable operation of our tlchptlc systems and the documentationstorage of operating param eters and results as required by the customer. Densitometric detection was performed with a camag tlc scanner 3 camag, muttenz, switzerland installed with wincats software.
Quantitative estimation of ascorbic acid by hptlc in. Tlcms online coupling, the comparatively new hyphenation of hptlc and mass spectrometry, has the potential to become an indispensable. Hptlc fingerprint profile of petroleum ether extracts from. Hptlc shows separation of components present in the chloroform, ethyl acetate and 90% alcoholic extract of the stem bark of ficus nervosa. The derivatization agent is simply included in the mobile phase advantageous.
Densitometric scanning of the developed plates in the reflectance mode at 555 nm using a camag tlc. The menus are selfexplanatory but in case of questions, help files. Srivastava editor the present edited book is the presentation of 18 indepth national and interna. Pdf high performance thin layer chromatography is an emerging. Samples were applied on the plate as a band with 4 mm width using camag 100. In thinlayer chromatography manual sample application with capillaries is usually. Hence, hptlc fingerprint profile has been developed for this plant. Whether it is managing of camag instruments or acquiring, storingretrieving, and evaluating of analytical data, all tasks can be performed with a few mouseclicks. It is a major advancement of tlc principle with short time duration and.
Hptlc principle, instrumentation and software abhishek gupta. Camag tlc visualizer 2 camag camag tlc hptlc, dbs, alox. Main difference of hptlc and tlc particle and pore size of sorbents. Camag tlc plate heater was used to preactivation of the hptlc plates and for drying the developed plates. Chromatography is a physical process of separation in which the components to be separated are distributed between two immiscible i. All the solvents used for hptlc analysis were obtained from merck, india.384 1140 328 179 1258 1378 1011 1298 1250 867 1049 894 1540 760 1494 128 216 1365 937 1240 406 180 418 985 1104 626 825 489 1187 695 866 1246 1067 41 1127